Deploying SDN architecture in Open Optical Transport Networks

Pro udrženı́ tempa s rostoucı́mi požadavky na přenosovou rychlost, latenci a bezpečnost je nutné zvážit současnou koncepci řı́zenı́ sı́tı́. Software-Defined Networking (SDN) je jedno z možných řešenı́, ke kterému telekomunikačnı́ průmysl směruje. Tato práce představuje současný stav Software-Defined Networking a zaměřuje se na vybraná open-source řešenı́ v oblasti SDN kontrolerů, jako je ONOS či OpenDaylight. Hlavnı́m cı́lem této části práce je vysvětlit, jak může SDN pomoci vyřešit rostoucı́ požadavky na rozšı́řenı́ automatizace v otevřených optických sı́tı́ch. Praktická část této práce je rozdělená do dvou oblastı́. V rámci prvnı́ oblasti jsem se zabýval rozšı́řenı́m funkčnosti SDN kontroleru pro umožněnı́ konfigurace a řı́zenı́ optických komunikačnı́ch zařı́zenı́. Hlavnı́m přı́nosem je implementace nových funkcionalit SDN driveru pro Nokia 1830 PSS (ROADM) a rozšı́řenı́ funkcionality driveru pro Nokia 1830 PSI-2T (optický transpondér). Ve druhé části práce jsem se zabýval problematikou korelace alarmů v otevřených optických sı́tı́ch. Výsledkem je funkce pro korelaci alarmů ve formě SDN aplikace, kterou jsem dále otestoval na emulovaných optických zařı́zenı́ch pro prokázánı́ funkčnosti celého konceptu.With the rising demands on the network throughput, latency and security, legacy control networking concepts should be reconsidered. Software-Defined Networking (SDN) is one of the possible solutions, to which telecommunication industry is moving. This work presents current state-of-the-art in Software-Defined Networking and focuses on some open-source solutions of SDN controllers, like ONOS and OpenDaylight. Main focus is to understand how SDN can help to solve increasing demand for broader automation in Optical Transport Networks. The practical section is divided in two parts. Within the first part I focused on extending functionality of SDN controller to facilitate more efficient configuration and control of optical network devices. Main contribution was to implement additional features to SDN drivers for Nokia 1830 PSS (ROADM) and extend functionality of Nokia 1830 PSI-2T (Optical Transponder) driver. Second part is dedicated to the Alarm Correlation problematic in open optical networks. We designed, developed an Alarm Correlation function as a SDN application then we tested it on emulated optical devices to prove the concept

Process control in the IoT concept

Tato práce se zabývá návrhem nízkonákladového systému v koncepci Internetu věcí pro dohled nad technologii galvanického vylučování kovů. Cílem je navrhnout funkční systém, který bude sbírat data a následně je zobrazovat. Součásti práce je též řešení problému spolehlivosti a bezpečnosti navrženého systému, včetně řešení problematiky ukládání dat v rámci omezené paměťové kapacity.This work deals with creating a system for controlling of electroforming process in IoT concept. The main aim is to create working system which can collect and show this data. Part of this work is also solving problem of reliability and security of created system and smart data storing in case of small memory capacity

Possibilities of combined surgical treatment of pyonecrotic lesions in the neuroischemic form of diabetic foot syndrome

Aim. To present results of combined surgical treatment of the ischemic form of diabetic foot syndrome complicated by pyonecrotic process. To show thatthe use of modern diagnostic tools and the choice of adequate treatment strategy permits to substantially reduce the number of above-the-knee amputations. Materials and methods. A total of 140 patients with diabetes mellitus and critical ischemia of lower extremities were under observation during 2004-2008.All of them had purulent and/or necrotic foot lesions. The patients were examined by X-ray, computed and magneto-resonance tomography of the feet,duplex scanning of lower leg vasculature, transcutaneous measurement of PO2, pelvic and lower leg arterial angiography. Results. A strategy of surgical treatment was developed to be used depending on the patients health status, clinical features and severity of pyonecroticprocesses in the foot, and the degree of involvement of the main blood vessels of lower extremities. Conclusion. Combination of endovascular surgery with various methods for plastic wound closure permits to extend the range of possibilities for the treatmentof high-risk patients, such as those with pyonecrotic lesions and neuroischemic form of diabetic foot syndrome. This approach opens up prospects fora significant decrease of percentage of above-the-knee amputations and improves the quality of life in these patients

Genetically encodable bioluminescent system from fungi

Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually oriented functions from attracting mates to scaring off predators. Half a dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which is found only in bacteria. Here, we report identification of the fungal luciferase and three other key enzymes that together form the biosynthetic cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite. Introduction of the identified genes into the genome of the yeast Pichia pastoris along with caffeic acid biosynthesis genes resulted in a strain that is autoluminescent in standard media. We analyzed evolution of the enzymes of the luciferin biosynthesis cycle and found that fungal bioluminescence emerged through a series of events that included two independent gene duplications. The retention of the duplicated enzymes of the luciferin pathway in nonluminescent fungi shows that the gene duplication was followed by functional sequence divergence of enzymes of at least one gene in the biosynthetic pathway and suggests that the evolution of fungal bioluminescence proceeded through several closely related stepping stone nonluminescent biochemical reactions with adaptive roles. The availability of a complete eukaryotic luciferin biosynthesis pathway provides several applications in biomedicine and bioengineering.This research was supported by Planta LLC and Evrogen JSC. IVIS imaging and animal experiments were carried out using the equipment of the Center for Collective Usage “Medical Nanobiotechologies” located in the Russian National Research Medical University. Experiments were partially carried out using the equipment provided by the Institute of Bioorganic Chemistry of the Russian Academy of Sciences Сore Facility (CKP IBCH; supported by Russian Ministry of Education and Science Grant RFMEFI62117X0018). T.G. and M.M.-H. acknowledge support from Spanish Ministry of Economy and Competitiveness Grant BFU2015-67107 cofounded by the European Regional Development Fund, European Research Council (ERC) Grant ERC-2012-StG-310325 under the European Union’s Seventh Framework Programme FP7/2007-2013, and the European Union’s Horizon 2020 Research and Innovation Programme under Marie Sklodowska-Curie Grant H2020-MSCA-ITN-2014-642095. F.A.K. acknowledges the support of HHMI International Early Career Scientist Program 55007424, the Spanish Ministry of Economy and Competitiveness (MINECO) Grants BFU2012-31329 and BFU2015-68723-P, MINECO Centro de Excelencia Severo Ochoa 2013-2017 Grant SEV-2012-0208, Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat’s Agency for Management of University and Research Grants Program 2014 SGR 0974, the Centres de Recerca de Catalunya Programme of the Generalitat de Catalunya, and ERC Grant 335980_EinME under the European Union’s Seventh Framework Programme FP7/2007-2013. H.E.W., A.G.O., and C.V.S. acknowledge support from São Paulo Research Foundation Fundação de Amparo à Pesquisa do Estado de São Paulo Grants 11/10507-0 (to H.E.W.), 10/11578-5 (to A.G.O.), and 13/16885-1 (to C.V.S.)